Deleted
Sample Discontinued
Experience: None
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Composite
Complete C

Part:BBa_K1088061:Design

Designed by: Patrick Rosendahl Andreassen   Group: iGEM13_SDU-Denmark   (2015-06-03)


Strong Promoter + Strong RBS + I-E Cascade


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1411
    Illegal XbaI site found at 3707
    Illegal XbaI site found at 3959
    Illegal SpeI site found at 541
    Illegal PstI site found at 3832
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1537
    Illegal SpeI site found at 541
    Illegal PstI site found at 3832
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2453
    Illegal BamHI site found at 133
    Illegal XhoI site found at 4783
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1411
    Illegal XbaI site found at 3707
    Illegal XbaI site found at 3959
    Illegal SpeI site found at 541
    Illegal PstI site found at 3832
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1411
    Illegal XbaI site found at 3707
    Illegal XbaI site found at 3959
    Illegal SpeI site found at 541
    Illegal PstI site found at 3832
    Illegal AgeI site found at 2208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 68


Design Notes

no


Source

See subparts

References